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KMID : 1007520000090050313
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Food Science and Biotechnology
2000 Volume.9 No. 5 p.313 ~ p.316
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Isolation and Identification of Azelaic Acid and 3,4-Dihydroxybenzoic Acid from Buckwheat Hull as Antimicrobial Substances
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Park, Keun Hyung
Cho, Jeong Yong/Kim, Hye Kyoung/Ma, Seung Jin/Moon, Jae Hak
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Abstract
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The methanol extract of buckwheat hull showed antimicrobial activity against bacteria. The extract was successively purified through solvent fractionation, silica gel adsorption chromatography, Sephadex LH-20 column chromatography, and octadecylsilane column chromatography. Further purification through high performance liquid chromatography gave two antimicrobial substances which were, respectively, identified as azelaic acid and 3,4-dihydroxybenzoic acid through MS and NMR analyses. The compounds showed antimicrobial activity against gram-positive and gram-negative bacteria at 200-500 ㎍ of concentration using the paper disc method.
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